Flexible endoscopy is an invaluable diagnostic and therapeutic procedure, which is performed in a variety of practice areas. As with all diagnostic and therapeutic procedures, there are always risks of complications, including infections, when endoscopy is performed. The risk of infection is not limited to the procedure. Personnel must ensure they are providing a safe, clean environment in all preprocedural, procedural, and postprocedural areas. Personnel must adhere to evidence-based guidelines when processing flexible endoscopes and related accessories, as any deviation can lead to increased risk of infection. To minimize the risk of infection, healthcare personnel must ensure that endoscopy equipment is designed and maintained properly, and that guidelines for processing are strictly followed. Personnel responsible for processing flexible endoscopes should receive initial and ongoing model-specific education and validation of competency. Personnel should also receive ongoing education in areas related to infection prevention and control, as well as the decontamination and sterilization of reusable medical equipment, instruments, and devices. Infection preventionists play a crucial role in ongoing risk assessments, audits to verify competencies, and compliance with manufacturer’s instructions for use and facility policies.