The perinatal period is the transition from pregnancy to postpartum and newborn phase of the reproductive life continuum. Pregnancy provides an opportunity to screen for diseases that impact the mother and her offspring. This period makes pregnant people and their newborns vulnerable to infections. Strategies to prevent hospital-acquired and postpartum infection can be employed. Vaccination for the mother before delivery and the newborn are included. The infection preventionist is a vital healthcare team member that promotes evidence-based infection control practices. Diligence to the environment, including cleaning and storing equipment, is paramount. Strategies for safely placing patients with potentially infectious diseases must be clear and employed when there is suspicion of transmission. Recommendations regarding patient placement and readmissions of postpartum patients and their newborns should be addressed. Standard policies and compliance with personal protective equipment (PPE)recommendations and dress codes are essential for breaking the chain of infection. This chapter will present the most recent evidence on infection prevention during the perinatal period.